ClonExpress II One Step Cloning (Vazyme, #C112) is a simple, fast, and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is added onto the insert through PCR. The insert and the linearized vector, with overlapped sequences of 15 bp-20 bp on both 5’- and 3’-end, respectively, are mixed and incubated with Exnase II at 37°C for 30 min. The cloning products can then be directly transformed to competent cells with a true positive rate > 95%. This kit contains Exnase II and an optimized buffer with a unique recombinant enhancer which significantly improves the recombination efficiency. In addition, Exnase II is compatible with the reaction systems of restriction enzyme digestion and PCR, which means that both the digestion products of vector and the PCR products of insert can be directly used for recombination without purification, significantly simplifying the procedures.Product Manual (pdf)

Advantages

• Easy, fast, and efficient.
• Directional cloning at any site on any vector.
• No need to consider the restriction enzyme cutting sites carried on inserts.
• Ligase-independent, true positive rate > 95%.
• Efficient cloning of fragments of 50 bp-10 kb.
• Linearized vector and PCR products can be used directly without purification.

When order, Cold Shipping option have to be selected, or your order may be delayed.

Selected Product Citations

1. Xing Y, et al. SLERT Regulates DDX21 Rings Associated with Pol I Transcription. Cell, 2017, 169(4):664-78. Pubmed ID:28475895
2. Wu N, et al. TBX6 null variants and a common hypomorphic allele in congenital scoliosis. N Engl J Med, 2015, 4793:341-50. Pubmed ID:25564734
3. Ge J, et al. Architecture of the mammalian mechanosensitive Piezo1 channel. . Pubmed ID: Nature, 2015, 527(7576):64-9. Pubmed ID:26390154
4. Zhang XO, et al. Complementary Sequence-Mediated Exon Circularization. Cell, 2014, 159(1):134-47. Pubmed ID:25242744
5. Zong Y, et al. Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. Nature Biotechnology, 2018. Pubmed ID:30272679
6. Li X, Wang Y, Liu Y, et al. Base editing with a Cpf1-cytidine deaminase fusion. Nature Biotechnology, 2018, 36(4):324-7. Pubmed ID:29553573

Mechanism

Storage

All components should be Store at -20°C.

Components

Related Products

1. 2 × Phanta Max Master Mix (Vazyme #P515)
2. Phanta Max Super-Fidelity DNA Polymerase (Vazyme #P505)
3. ClonExpress Ultra One Step Cloning Kit (Vazyme, #C115)
4. ClonExpress MultiS One Step Cloning Kit (Vazyme, #C113)
5. Mut Express II Fast Mutagenesis Kit V2 (Vazyme, #C214)
6. Mut ExpressMultiS Fast Mutagenesis Kit V2 (Vazyme, #C215)

Cellagen利用广泛的研究以及我们在干细胞生物学，药物化学和产品开发方面的专业知识，Cellagen长期以来一直为专业OEM产品提供世界领先的公司，为一线干细胞研究奠定了基础。

现在，Cellagen扩展了其最佳产品系列，并直接向研究界提供。Cellagen与世界领先的科学家有着密切的联系，并且始终认识到高标准科学研究的未满足需求。