ProductDescription
Toachievethefullpotentialofstemcellsinregenerativemedicineanddiseasemodeling,highconversionefficiencyfromhESCs/hiPSCstoneuralcellsiscrucial.Asupplementofthissmallmoleculecocktailincellculturemediasimplifiestheprocessandincreasestheproductivityofneuralandneuronalcellgeneration.
Repro-nLS(alsonamedasLSBinreference1)isacombinationoftwosmall-moleculeinhibitors(LDN193189andSB431542).UndertheRepro-nLScondition,bothBMP(ALK2/3)andTGF-beta1(ALK4/5/7)signalingpathwaysareinhibitedbyLDN193189andSB431542respectively.hESCs/hiPSCscanbeefficientlyconvertedintoneuralprecursorcellsinamonolayerfashionunderthisdualSMADinhibition.Morethan80%ofhESCsarerapidlyanduniformlyconvertedintoPax6+neuralprecursorcells.ThiscocktailcanbefurthercombinedwithotherpatterningfactorstoreADIlygeneratesubtypespecificneuralandneuronalcells1-3,suchasnociceptorswhenusedwith3i1(SU5402,CHIR99021andDAPT)(SeeRepro-nLSB3i).
Technicalinformation:
| CocktailFormulation: 1000xDMSOstocksolution | 0.1mMofLDN193189(BMPinhibitor),and 10mMofSB431542(TGF-ß1inhibitor). | |
| Purity: | >98%(foreachcompound) |
ShippingCondition:Theproductisshippedinaglassvialatambienttemperature.
Storage:StoreDMSOsolutionat-20oC.
Usage:Add/DiluteRepro-nLSstocksolutionat1:1000intoyourhESC/iPSCbasalmediatoreconstitute1xmediaforneuraldifferentiation.
Reference:
| 1. | ChambersSM,etal.,Combinedsmall-moleculeinhibitionacceleratesdevelopmentaltimingandconvertshumanpluripotentstemcellsintonociceptors.NatBiotechnol.201230(7):715-20. |
| 2. | ChambersSM,etal.HighlyefficientneuralconversionofhumanESandiPScellsbydualinhibitionofSMADsignaling.NatBiotechnol.200927(3):275-80. |
| 3. | YuPB.,etal.BMPtypeIreceptorinhibitionreducesheterotopicossification.NatMed.200814(12):1363-9. |
Cellagen利用广泛的研究以及我们在干细胞生物学，药物化学和产品开发方面的专业知识，Cellagen长期以来一直为专业OEM产品提供世界领先的公司，为一线干细胞研究奠定了基础。
现在，Cellagen扩展了其最佳产品系列，并直接向研究界提供。Cellagen与世界领先的科学家有着密切的联系，并且始终认识到高标准科学研究的未满足需求。
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