ProductDescription
Datahasshowndirecttransdifferentiationbetweendifferentgermlayerswhentransfectedwithacocktailofdefinedtranscriptionfactorgenes1,3.Asupplementofthissmallmoleculecocktailincellculturemediasimplifiestheprocessandincreasestheproductivityofneuralandneuronalcellgeneration.
Repro-nLSCisacocktailofthreesmallmoleculeinhibitors.ItincludesLDN193189,SB431542andCHIR99021.UsingthecombinationofsynergisticSMADinhibitionthroughBMPandTGF-betapathwaystogetherwithspecificGSK-3betainhibition,arecentstudyhaspresentedthatpostnatalhumanfibroblasts(HPF),transducedwithAscl1andNgn2,efficientlyconvertedintofunctionalneuron-likecellswithyieldsupto>200%andneuronalpuritiesupto>80%.Inadditiontotheconversionofmesenchymalcellstoinducedneurons(iNs),thismethodhasalsobeentestedintheconversionofothersomaticcelltypes(eg.Cordblood-derivedstemcells)toiNs3.
Technicalinformation:
| CocktailFormulation: 1000xDMSOstocksolution | 0.5mMofLDN193189(BMPinhibitor), 10mMofSB431542(TGF-ß1inhibitor),and 2mMofCHIR99021(GSK3ßinhibitor) | |
| Purity: | >98%(foreachcompound) |
ShippingCondition:Theproductisshippedinaglassvialatambienttemperature.
Storage:StoreDMSOsolutionat-20oC.
Usage:DiluteRepro-nLSCstocksolution(1000x)at1:1000intoyourreprogrammingmediatoinitiateneuronalconversion.
Reference:
| 1. | VierbuchenT,etal.Directconversionoffibroblaststofunctionalneuronsbydefinedfactors.Nature.2010.463(7284):1035-41. |
| 2. | ChambersSM,etal.HighlyefficientneuralconversionofhumanESandiPScellsbydualinhibitionofSMADsignaling.NatBiotechnol.200927(3):275-80. |
| 3. | LadewigJ.,etal.Smallmoleculesenablehighlyefficientneuronalconversionofhumanfibroblasts.NatMethods.2012.9(6):575-8. |
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